Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 20;13:176. doi: 10.1186/s13068-020-01815-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Effects of gene disruption on cell growth, mannitol, and erythritol synthesis. a Molecular identification of 7 genes disruption (Ku70, YlAraDH1, YlAraDH2, YlMDH1, YlMDH2, YlXDH, YlEYD1 genes), no corresponding band can be amplified from the disruptants, but the band can be amplified for wild-type control strain (Y. lipolytica CGMCC7326, ery929), indicating Ku70, YlAraDH1, YlAraDH2, YlMDH1, YlMDH2, YlXDH, or YlEYD1 genes were disrupted individually. b Growth curves of different disruptants on liquid medium containing 300 g/L glucose. c Effects of various gene disruption on erythritol and mannitol biosynthesis, only mdh2 gene disruption can result in no mannitol synthesis. d, e The HPLC chromatography of wild-type strain and mdh2 gene disruption, showing no mannitol when the mdh2 gene was disrupted