Table 3.
Summary of fermentation profiles of engineered strain HCY118 and wild-type control strain at 30, 33, 34, 35 °C
| Strains | Temp | Erythritol (titer: g/L) |
Mannitol (titer: g/L) |
Qery (g/L h) | Yery (g/g glucose) | T (h) | Rglu (g/L h) | OD600 | DCW (g/L) | CPery (g/g dcw h) |
|---|---|---|---|---|---|---|---|---|---|---|
|
CGMCC7326 (wild-type control) |
30 °C | 166 ± 5.5 | 7.5 ± 0.5 | 1.73 | 0.55 | 96 | 3.13 | 32.4 ± 1.5 | 8.1 | 0.22 |
| 33 °C | 145 ± 5.5 | 12.4 ± 0.5 | 1.21 | 0.48 | 120 | 2.5 | 28.8 ± 1.5 | 7.2 | 0.17 | |
| HCY118 | 30 °C | 189 ± 6 | 0 | 1.97 | 0.63 | 96 | 3.13 | 30.5 ± 1.5 | 7.6 | 0.26 |
| 33 °C | 190 ± 6 | 0 | 1.97 | 0.63 | 96 | 3.13 | 32.6 ± 1.5 | 8.2 | 0.24 | |
| 34 °C | 162 ± 5.5 | 0 | 1.2 | 0.54 | 135 | 2.22 | 24.4 ± 1.5 | 6.1 | 0.16 | |
| 35 °C | 132 ± 4 | 0 | 0.78 | 0.44 | 170 | 1.76 | 20.6 ± 1.5 | 5.3 | 0.11 |
Qery: productivity; Yery: erythritol yield; Rglu: glucose consumption rate; DCW: dry cell weight; CPery: cell production rate