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CTCs classification criteria
| Type | Red spot | Green spot | Gray spot | DAPI |
|---|---|---|---|---|
| CTCs | ||||
| I | + | – | – | + |
| II | + | + | – | + |
| III | – | + | – | + |
Type I: epithelial CTCs, red fluorescence
Type II: epithelial-mesenchymal CTCs, red and green fluorescence
Type III: mesenchymal CTCs, green fluorescence
