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. 2020 Oct 20;39:221. doi: 10.1186/s13046-020-01703-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — VEGFA derived from hypoxic PMCs facilitates GC cell adhesion and migration via VEGFR1. a The effect of exogenous VEGFA on cell migration was observed in the presence of Apatinib or Bevacizumab. Representative photographs of migratory cells are shown. Scale bar represents 100 μm. Error bars represent SD of the mean. *P < 0.05. b Western blot analysis of VEGFR1 protein levels in various GC cells. c Survival analysis for different VEGFR1 expression levels using the KM-Plotter database of GC. d MGC-803 sgRNA-VEGFR1 and sgRNA-NC cells were intraperitoneally inoculated. The metastatic nodules (green arrows) were analyzed after 20 days (N = 5 per group). Representative data are shown. ****P < 0.0001. e and f MGC-803 cells were exposed to CM from hypoxic PMCs or exogenous VEGFA, synchronously with knockout of VEGFR1 using sgRNA-FLT1. Representative photographs of adherent and migratory cells are shown. Scale bar represents 100 μm. Error bars represent SD of the mean. *P < 0.05. **P < 0.01. ***P < 0.001