Fig. 4.

miR-495 targets YTHDF2 and suppresses its expression. a, Predicted binding sites between miR-495 and YTHDF2 via bioinformatics analysis. b, The mRNA expression of YTHDF2 in PCa and adjacent normal tissues detected by RT-qPCR. c, Correlation between miR-495 and YTHDF2 in PCa tissues and adjacent normal tissues of the patients. d, Western blot analysis of YTHDF2 protein in cancer and adjacent normal tissues of the patients. e, The binding of miR-495 to the mRNA of YTHDF2 confirmed by dual luciferase reporter assay. f and i, miR-495 expression in miR-495 mimic- or miR-495 inhibitor-transfected cells detected by RT-qPCR. G and j, YTHDF2 mRNA expression in miR-495 mimic- or miR-495 inhibitor-transfected cells detected by RT-qPCR. h and k, Western blot analysis and quantification of YTHDF2 protein in miR-495 mimic- or miR-495 inhibitor-transfected cells. * p < 0.05 vs. adjacent normal tissues, or cells transfected with mimic NC or inhibitor NC. Data in panel B and D were analyzed using paired t-test, and those in panel E-K were analyzed using unpaired t-test