Fig. 4.

The effect of daphnetin on the proliferation of HaCaT keratinocytes stimulated with M5. a HaCaT keratinocytes were seeded in 96-well plates and treated with daphnetin (Dap, 20 μM) or DMSO (Ctl) for 2 h, and subsequently stimulated with M5 cytokines (2.5 ng/ml) or not for 24–96 h. CCK-8 was used to measure cell viability. *P < 0.05. **P < 0.01. b HaCaT keratinocytes were seeded in 12-well plates and treated with daphnetin (Dap, 20 μM) or DMSO for 2 h, and subsequently stimulated with M5 cytokines (2.5 ng/ml) or not for 72 h. Cells were harvested and RNA was extracted for qRT-PCR analysis of KRT6 mRNA level. GAPDH served as an internal reference. *P < 0.05. DMSO (Ctl), DMSO + M5 (M5), daphnetin + M5 (M5 + Dap)