Figure 2.

Effect of NF-κB inhibitors on IL-1β-induced COX-2 expression. Synovial fibroblasts were pretreated with or without the NF-κB inhibitors BAY11-7082 (10 μM) and TPCA-1 (10 µM), for 1 h and subsequently stimulated with IL-1β (100 pM). After stimulation for 6 or 48 h, we analyzed COX-2 mRNA levels (A) and prostaglandin E₂ release (B). The inhibitors decreased IL-1β-induced COX-2 expression and prostaglandin E₂ release. Results have been represented as mean ± SE from biological triplicates. *P < 0.05.