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. 2020 Oct 7;11:579266. doi: 10.3389/fimmu.2020.579266

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Copyright © 2020 Nakano, Kitanaka, Namba, Kitanaka, Suwabe, Konno, Yamazaki, Nakayama and Sugiya

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of IL-1β-induced COX-2 expression in synovial fibroblasts depleted of p65 and p105. (A) Protein levels of total p65 (t-p65), total p105 (t-p105), and β-actin (internal standard) were detected by immunoblotting in cells transfected with p65, p105, or scramble siRNAs (control). (B) The relative levels of t-p65 and t-p105 as compared to the levels in the control samples. (C) Cells transfected with p65, p105, or scramble siRNAs were stimulated with IL-1β for 6 h. Depleting cells of p65 and p105 inhibited IL-1β-induced COX-2 expression. Results have been represented as mean ± SE from biological triplicates. *P < 0.05. Cell lysates (10 μg protein) were used for immunoblotting.