Fig. 2.

Optimising pre-treatment of patient’s swab samples for direct usage in RT-LAMP. Positive sample 1–5 and Negative sample 1–5 were pre-heated at 80 °C, 85 °C, 90 °C and 95 °C for 5 min before being directly pipetted into RT-LAMP for SARS-CoV-2 targeting OFR8 (a) and N (b). All experiments were performed three times in duplicates. Amplification Plots show representative results for six measurements per sample