Table 3.
Quantification Cycle (Cq) values of RT-qPCR compared to detection times of RT-LAMP detecting SARS-CoV-2 in pharyngeal swab samples
| Pharyngeal swab | Cq value RT-qPCR Orf1a |
Detection time RT-LAMP ORF8 |
Detection time RT-LAMP N |
Detection time RT-LAMP RNase P (IC) |
|---|---|---|---|---|
| Positive 1 | 18.40 | 9.55 | 12.67 | 22.78 |
| Positive 2 | 24.13 | 11.48 | 15.24 | 23.22 |
| Positive 3 | 28.97 | 11.93 | 17.84 | 19.34 |
| Positive 4 | 29.26 | 14.19 | 17.39 | 20.30 |
| Positive 5 | 30.47 | 15.05 | 19.34 | 20.98 |
| Negative 1 | 20.97 | |||
| Negative 2 | 21.06 | |||
| Negative 3 | 21.69 | |||
| Negative 4 | 23.96 | |||
| Negative 5 | 15.18 |
Ten pharyngeal swab samples were routinely tested for COVID-19 using ampliCube Coronavirus SARS-CoV-2 kit acquired from Mikrogen Diagnostik, where Orf1a serves as specific target for SARS-CoV-2. Positive samples 1–5 and Negative samples 1–5 were pre-heated at 90 °C for 5 min and directly tested for SARS-CoV-2 in RT-LAMP. The targets ORF8 and N detect SARS-CoV-2, while RNase P is used as internal control (IC) for proof of human material in the swab