Figure 1.

Effect of 2,3-DCPE on S phase arrest and p-H2A.X expression in the DLD-1 cell line. Cells were treated with 20 μM 2,3-DCPE for 8, 10, 12, 14, 16 or 18 h. (A) Cell cycle distribution is presented for each experimental condition. (B) p-H2A.X and total H2A.X levels in the cellular extracts were determined by western blotting with specific antibodies. β-actin was used as an internal control. 2,3-DCPE, 2[[3-(2,3-dichlorophenoxy)propyl]amino]ethanol; p-, phosphorylated; H2A.X, H2A histone family member X.