Figure 3.

Effect of ATM inhibitors on the 2,3-DCPE-induced cell cycle arrest in the S phase. (A) Cell cycle distribution was determined by flow cytometry assays and (B) the quantitative analysis is presented in the bar chart. Data are expressed as the mean ± standard deviation. **P<0.01 vs. the 2,3-DCPE group. (C) Western blotting was performed to investigate the p-Chk1 and Cdc25A expression after cells were treated with 2,3-DCPE and wortmannin or caffeine. 2,3-DCPE, 2[[3-(2,3-dichlorophenoxy)propyl]amino]ethanol; p-, phosphorylated; Chk, checkpoint kinase; ATM, ataxia-telangiectasia mutated; Cdc25A, M-phase inducer phosphatase 1; DMSO, dimethyl sulfoxide.