KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PE/Cy7 anti-human CD38 antibody | Biolegend | Cat# 303516, RRID:AB_2072782 |
| Mouse IgG1 kappa Isotype Control PE / Cyanine 7 | Biolegend | Cat# 400126, RRID:AB_326448 |
| Pacific Blue anti-human CD45RA antibody | Biolegend | Cat# 304118, RRID:AB_493657 |
| APC/Cyanine7 anti-human CD197 (CCR7) antibody | Biolegend | Cat# 353212, RRID:AB_10916390 |
| Alexa Fluor® 700 anti-human CD8a antibody | Biolegend | Cat# 301028, RRID:AB_493745 |
| PE anti-human CD107a (LAMP-1) antibody | Biolegend | Cat# 328608, RRID:AB_1186040 |
| CD279 (PD-1) Monoclonal Antibody (MIH4) | eBioscience | Cat# 17-9969-42, RRID:AB_10718533 |
| Alexa Fluor® 700 anti-human CD8a antibody | Biolegend | Cat# 301028, RRID:AB_493745 |
| Alexa Fluor® 700 anti-human Granzyme A antibody | Biolegend | Cat# 507210, RRID:AB_961343 |
| Granzyme B antibody | eBioscience | Cat# 563388, RRID:AB_2738174 |
| Perforin antibody | BD Biosciences | Cat# 563762, RRID:AB_2738409 |
| PE anti-human CD107a (LAMP-1) antibody | Biolegend | Cat# 328608, RRID:AB_1186040 |
| PE anti-human IFN-gamma antibody | Biolegend | Cat# 502509, RRID:AB_315234 |
| APC anti-human CD3 antibody | Biolegend | Cat# 300439, RRID:AB_2562045 |
| Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Alexa FluorR 647 Conjugate) | Cell Signaling Technology | Cat# 12158S, RRID:AB_2797834 |
| Acetylated-Lysine Antibody | Cell Signaling Technology | Cat# 9441S, RRID:AB_331805 |
| Ezh2 (AC22) Mouse mAb | Cell Signaling Technology | Cat# 3147S, RRID:AB_10694383 |
| Runx3 human antibody PE | BD Biosciences | Cat# 564814, RRID:AB_2738969 |
| EOMES Monoclonal Antibody (WD1928), PE | eBioscience | Cat# 12-4877-42, RRID:AB_2572615 |
| Alexa Fluor® 647 anti-T-bet antibody | Biolegend | Cat# 644804, RRID:AB_1595466 |
| FITC anti-human CD223 (LAG-3) antibody | Biolegend | Cat# 369308, RRID:AB_2629751 |
| APC anti-human HLA-DR antibody | Biolegend | Cat# 307610, RRID:AB_314688 |
| PE anti-human TIGIT (VSTM3) antibody | Biolegend | Cat# 372704, RRID:AB_2632730 |
| PE anti-human CD158 (KIR2DL1/S1/S3/S5) antibody | Biolegend | Cat# 339505, RRID:AB_2130376 |
| Anti-KAT3B / p300 antibody | abcam | Cat# Ab10485, RRID:AB_297224 |
| IL-17A Monoclonal Antibody (eBio64DEC17) | Cat# 17-7179-42, RRID:AB_1582221 | |
| FITC anti-human IL-4 antibody | Biolegend | Cat# 500807, RRID:AB_315126 |
| PE anti-human IL-13 antibody | Biolegend | Cat# 501903, RRID:AB_315198 |
| IL-10 Monoclonal Antibody (JES3–9D7) | eBioscience | Cat# 50-7108-42, RRID:AB_11149363 |
| anti-human CD3 (OKT3) | Bio X Cell | Cat# BE0001–2, RRID:AB_1107632 |
| LEAF(TM) Purified anti-human CD28 antibody | Biolegend | Cat# 302923, RRID:AB_2291210 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| LentiCas9-Blast | ATCC | Cat# 52962 |
| pSPgRNA | ATCC | Cat# 47108 |
| GSK126 | Cayman Chemical | Cat# 15415 |
| EX527 | Sigma-Aldrich | Cat# E7034 |
| Pacific Orange Succinimidyl Ester | Thermo Fisher Scientific | Cat# P30253 |
| Critical Commercial Assays | ||
| NAD+/NADH quantitation kit | Sigma-Aldrich | Cat# MAK037–1KT |
| BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit | BD Biosciences | Cat# 554714 |
| Pierce Classic Magnetic IP/Co-IP Kit | Thermo Fisher Scientific | Cat# 888804 |
| RosetteSep Human CD8+ T Cell Enrichment Cocktail | Stem Cell technologies | Cat# 15063 |
| RosetteSep Human T Cell Enrichment Cocktail | Stem Cell technologies | Cat# 15061 |
| Cell Line Nucleofector™ Kit V | Lonza | Cat# VVCA-1003 |
| Human T Cell Nucleofector™ Kit | Lonza | Cat# VPA-1002 |
| RNeasy Plus Mini kit | QIAGEN | Cat# 74134 |
| Lymphocyte Separation Medium | Corning | Cat# 25–072-CV |
| Deposited Data | ||
| Raw and analyzed ATAC-seq data | This paper | GEO: GSE141197 |
| Experimental Models: Cell Lines | ||
| Jurkat E6.1 | ATCC | CLS Cat# 300223 RRID:CVCL_0367 |
| TALL-104 | ATCC | CRL-11386 |
| P815 | ATCC | Cat# TIB-64 |
| Oligonucleotides | ||
| CD38 Hs01120071_m1 | Thermo Fisher Scientific | Cat# 4331182 |
| TBX21 Hs00894392_m1 | Thermo Fisher Scientific | Cat# 4331182 |
| EOMES Hs00172872_m1 | Thermo Fisher Scientific | Cat# 4331182 |
| RUNX3 Hs01091094_m1 | Thermo Fisher Scientific | Cat# 4331182 |
| GZMB Hs00188051_m1 | Thermo Fisher Scientific | Cat# 4331182 |
| GZMA Hs00989184_m1 | Thermo Fisher Scientific | Cat# 4331182 |
| PRF1 Hs00169473_m1 | Thermo Fisher Scientific | Cat# 4331182 |
| GUSB Hs00939627_m1 | Thermo Fisher Scientific | Cat# 4331182 |
| TBP Hs00427620_m1 | Thermo Fisher Scientific | Cat# 4331182 |
| Recombinant DNA | ||
| CD38 cDNA ORF Clone, Human, C-Myc tag | Sino Biological | Cat# HG10818-CM |
| pmaxGFP | Lonza | Cat# VPA-1002 |
| pCMV3-C-Myc Negative Control Vector | Sino Biological | Cat# CV014 |
| Software and Algorithms | ||
| Kaluza software | Beckman Coulter | version 1.5a |
| Prism version 6 | GraphPad | https://www.graphpad.com/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| CHOPCHOP | N/A | http://chopchop.cbu.uib.no/ |