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Comparison of the percentages of viable, early apoptotic, late apoptotic and necrotic cells in 2D monolayer or 3D spheroids MDA-MB-231 cells treated with different concentrations of CEP or TET. 2D- or 3D-cultured MDA-MB-231 cells treated with CEP or TET were categorized into viable (white), early apoptotic (light grey), late apoptotic (dark grey) and necrotic (black) cells after staining with Annexin V and PI analyzed via flow cytometry. C/CEP, cepharanthine; T/TET, tetrandrine.
