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. 2020 Oct 6;20(6):331. doi: 10.3892/ol.2020.12191

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Copyright: © Kiyomi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 9. — Fluorescence intensities and morphological examinations of spheroids of 3D MCF-7 cells treated with different concentrations of CEP or TET. Fluorescence intensity of (A) calcein and (B) propidium iodide in the spheroids. (C) Spheroid area and (D) spheroid roundness of 3D MCF-7 cells treated with CEP or TET. The intensity of calcein (cytoplasm) and propidium iodide (dead cells) fluorescence were detected after 15 min of incubation using an Operetta CLS (PerkinElmer, Inc.). The spheroid area and roundness were calculated using the same instrument. The data are shown as the mean + SD of 4 independent experiments. A roundness value of 1 corresponds to complete roundness, and lower values indicate the deformation of the spheroid. *P<0.05 vs. control analyzed via one-way ANOVA and Dunnett's multiple comparisons test. C/CEP, cepharanthine; T/TET, tetrandrine.