FIG 3.

ABI-H0731 inhibits cccDNA establishment in de novo-infected cells. HBV infection assays in HepG2-NTCP cells and PHHs and Southern blot analysis of cccDNA and capsid DNA are described in Materials and Methods. (A) Treatment schedule. HepG2-NTCP cells and PHH cells were infected with HBV for 1 or 3 h for capsid DNA or cccDNA detection, respectively, and an MOI of 2,500 was used for capsid DNA analysis, whereas MOIs of 500 and 1,000 were used for cccDNA detection in HepG2-NTCP cell and PHH infection assays, respectively. After infection, the cells were treated with ABI-H0731 or ETV for 3 h or 4 days for capsid DNA or cccDNA detection, respectively. (B) Southern blot analysis of capsid DNA and cccDNA extracted from HBV-infected HepG2-NTCP cells and PHHs. Band density was quantified using ImageJ software.