Figure 2.

Transfection reagent selection for U937 and human macrophages. 20 nM Bax siRNA was transfected with 3 different doses of each transfection reagent (the amount recommended by the manufacturer, +25% and −25% of the dose recommended by the manufacturer). For clarity and ease of presentation, the best two kill curves for the transfection reagents closest to the ‘no treatment curve’ are shown. If the best two curves selected for a transfection reagent were very close to each other, however, only one curve is shown. Representative graph for the time-course cell death of PMA-differentiated U937 cells (A) and primary human MDMs (B) pretreated with Bax siRNA 24 hr before treatment with RESV. (C) The cytotoxicity comparison between DharmaFECT 3 and Lipofectamine 2000 in primary human MDMs. 20 nM BLOCK-iT™ Alexa Fluor® Red Fluorescent control siRNA was transfected with either 1.0 μl DharmaFECT 3 or 6.0 μl Lipofectamine 2000 for 16 hr following which the cells were imaged under fluorescence microscope.