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Histological evaluation of ALS submitted to ex vivo blood perfusion tests. H&E staining revelated whole blood vessel congestion (black arrows) in ALS perfused with rat blood diluted in PBS after 1 h of continuos perfusion (a). No blood vessel congestion or clotting were detected in ALS perfused with rat blood diluted in HTK solution + Ringer’s Lactate and heparin after 1 h of continuous perfusion (b). 10x, Scale bars: 100 µm.
