Figure 8.

Imaging experiments for cell permeability of the stapled and linear peptides labeled with fluorescein to HeLa cells. Each panel is divided into three sections as follows: upper, differential interference contrast (DIC) image; middle, fluorescein emission; lower, merged image. The peptide concentration is 5 μM. After addition of peptides, cells were incubated at 37 °C for 30 min under 5% CO₂ atmosphere. After washing three times with HBSS buffer, the fluorescent imaging was conducted by Fluoview FV10i confocal microscopy systems (Olympus). Orange bars in the panels represent 10 μm. The fluorescein-labeled peptides contain fluorescein–GABA instead of acetyl in the N-terminus.