Fig. 1.

Adipose tissue specific regulation of DUSP1 and inflammatory mediators during the development of obesity under conditions of genetic and diet-induced obesity. Total RNA was prepared from white adipose tissue (WAT) harvested during development of genetic obesity at (A) 6 and (B) 10 weeks of age from male B6-ob/ob mice and their lean litter mates. For development of diet-induced obesity (DIO), C57BL/6J males were given ad libitum access to a high fat diet (HFD; 60% kcal) starting at 6 weeks of age. Control males were fed a control low fat diet (LFD) containing 10% kcal from fat and the same protein content as the HFD. RNA was isolated from WAT at (C) 18 and (D) 24 weeks of age. Relative DUSP mRNA abundance was measured via qRT-PCR and statistical significance determined by student’s t-test (P<0.05). All data were normalized to 18S rRNA and expressed relative to lean littermates.