Fig. 3.

Inhibition of DUSP1 enhances ERK and p38 phosphorylation during early adipocyte differentiation. (A) PAs were pretreated (30 min) in the absence or presence of 1 ng/ml Actinomycin D (AD) prior to MDI stimulation. Cell lysates were collected over time and protein expression of phosphorylated ERK and p38 along with DUSP1 and GAPDH analyzed via immunoblotting. (B) In addition, PAs were pretreated (30 min) in the presence or absence of 1 μM triptolide (TRP) prior to MDI stimulation. Cell lysates were collected over time post-MDI and protein expression of phosphorylated ERK and p38 along with DUSP1 and α-Tubulin examined via immunoblotting.