TABLE 1.
DUSP and inflammatory genes analyzed in this study
| Symbol | Name/alias | Accession | ABI number | CT | |||||
|---|---|---|---|---|---|---|---|---|---|
| Dual specificity phosphatases | GP | MKB | NLS | NES | EJP | ||||
| Dusp1 | MKP-1,hVH1 | I | ● | ● | NM_013642 | Mm00457274_g1 | 24 | ||
| Dusp2 | PAC-1 | I | ● | ● | NM_010090 | Mm00839675_g1 | 30 | ||
| Dusp4 | MKP-2, hVH2 | I | ● | ● | NM_176933 | Mm00723761_m1 | 25 | ||
| Dusp5 | hVH3 | I | ● | ● | NM_001085390 | Mm01266104_m1 | 27 | ||
| Dusp6 | MKP-3, rVH6 | II | ● | ● | E | NM_026268 | Mm00650255_g1 | 24 | |
| Dusp7 | MKP-X | II | ● | ● | E | NM_153459 | Mm00463228_m1 | 27 | |
| Dusp9 | MKP-4 | II | ● | ● | E | NM_029352 | Mm00512646_m1 | 27 | |
| Dusp8 | M3/6, hVH5 | III | ● | ● | ● | J/P | NM_008748 | Mm00456230_m1 | 27 |
| Dusp10 | MKP-5 | III | ● | J/P | NM_022019 | Mm00517678_m1 | 24 | ||
| Dusp16 | MKP-7, MKP-M | III | ● | ● | ● | J/P | NM_130447 | Mm00459935_m1 | 25 |
| Inflammatory genes | |||||||||
| IL-6 | Interleukin-6 | NM_031168 | Mm99999064_m1 | 32 | |||||
| TNFα | Tumor necrosis factor-alpha | NM_013693 | Mm99999068_m1 | 28 | |||||
| Reference gene | |||||||||
| 18S | 18 Ribosomal RNA | X03205 | 4342930E | 9 | |||||
DUSP group (GP), MAPK binding domain (MKB), nuclear localization sequence (NLS), nuclear export sequence (NES), ERK (E), JNK (J), p38 (P), threshold cycle (CT) measured in lean adipose tissue.