Table 1. Multiple targets Cas9 candidates for AtCBF genes.
All possible genome targets and offtargets (with ARES-GT thresholds: L0 = 4 and L1 = 3) of each candidate are listed with indication of genome coordinates (TAIR v10) and whether it corresponds to a CBF gene. In alignments, black boxes mark mismatches and a space separates PAM (NGG or NAG) from sequence. Differences in the “N” position in the PAM are not marked.
| Candidate ID | Targets + Offtargets (L0 = 4, L1 = 3) | |||||
|---|---|---|---|---|---|---|
| A. thaliana | Gene | chrom | start | end | sense | sequence |
| Cas9AtCBF1_014 | AtCBF2 | 4 | 13015820 | 13015842 | + | AGCACGAGCTGCCATCTCAG CGG |
| AtCBF1 | 4 | 13022305 | 13022327 | + | AGCACGAGCTGCCATCTCAG CGG | |
| AtCBF3 | 4 | 13018737 | 13018759 | + | AGCTCGAGCTGCCATCTCAG CGG | |
| Cas9AtCBF1_015 | AtCBF2 | 4 | 13015825 | 13015847 | + | GAGCTGCCATCTCAGCGGTT TGG |
| AtCBF1 | 4 | 13022310 | 13022332 | + | GAGCTGCCATCTCAGCGGTT TGG | |
| Cas9AtCBF1_018 | AtCBF2 | 4 | 13015920 | 13015942 | + | TGACGAACTCCTCTGTAAAT TGG |
| AtCBF1 | 4 | 13022405 | 13022427 | + | TGACGAACTCCTCTGTAAAT TGG | |
| AtCBF4 | 5 | 21117612 | 21117634 | + | TGACGAACTCCTCTGTAAAT CGG | |
| AtCBF3 | 4 | 13018837 | 13018859 | + | CGACGAACTCCTCTGTATAT TGG | |
| Cas9AtCBF1_019 | AtCBF2 | 4 | 13015921 | 13015943 | + | GACGAACTCCTCTGTAAATT GGG |
| AtCBF1 | 4 | 13022406 | 13022428 | + | GACGAACTCCTCTGTAAATT GGG | |
| ---- | 1 | 1597274 | 1597296 | + | CACAATCTCCTCTGTAAATT CAG | |
| AtCBF3 | 4 | 13018838 | 13018860 | + | GACGAACTCCTCTGTATATT GGG | |
| Cas9AtCBF1_051 | AtCBF2 | 4 | 13015738 | 13015760 | - | CCG GGATTCGTAGCCGCCAAGCC |
| AtCBF1 | 4 | 13022223 | 13022245 | - | CCG GGATTCGTAGCCGCCAAGCC | |
| Cas9AtCBF1_056 | AtCBF2 | 4 | 13015831 | 13015853 | - | CCA TCTCAGCGGTTTGGAAAGTC |
| AtCBF1 | 4 | 13022316 | 13022338 | - | CCA TCTCAGCGGTTTGGAAAGTC | |
| AtCBF3 | 4 | 13018748 | 13018770 | - | CCA TCTCAGCGGTTTGAAATGTT | |
| Cas9AtCBF1_061 | AtCBF2 | 4 | 13015900 | 13015922 | - | CCC ACTTACCGGAGTTTCTTTGA |
| AtCBF1 | 4 | 13022385 | 13022407 | - | CCC ACTTACCGGAGTTTCTTTGA | |
| AtCBF3 | 4 | 13018817 | 13018839 | - | CCC ACTTACCGGAGTTTCTCCGA | |
| Cas9AtCBF1_062 | AtCBF2 | 4 | 13015901 | 13015923 | - | CCA CTTACCGGAGTTTCTTTGAC |
| AtCBF1 | 4 | 13022386 | 13022408 | - | CCA CTTACCGGAGTTTCTTTGAC | |
| AtCBF3 | 4 | 13018818 | 13018840 | - | CCA CTTACCGGAGTTTCTCCGAC | |
| Cas9AtCBF1_063 | AtCBF2 | 4 | 13015908 | 13015930 | - | CCG GAGTTTCTTTGACGAACTCC |
| AtCBF1 | 4 | 13022393 | 13022415 | - | CCG GAGTTTCTTTGACGAACTCC | |
| ---- | 2 | 6123419 | 6123441 | - | CCC GACTTTCTTTGAAGAACTCC | |
| Cas9AtCBF1_064 | AtCBF2 | 4 | 13015929 | 13015951 | - | CCT CTGTAAATTGGGTGACGAGT |
| AtCBF1 | 4 | 13022414 | 13022436 | - | CCT CTGTAAATTGGGTGACGAGT | |
| AtCBF3 | 4 | 13018846 | 13018868 | - | CCT CTGTATATTGGGTGACGAGT | |
| ---- | 1 | 4290740 | 4290762 | - | CCT CTGTAAACTGGGTGACGTGT | |
| ---- | 1 | 23368054 | 23368076 | - | CCT CTGTAGATTGGGTGACGTGT | |
| AtCBF4 | 5 | 21117621 | 21117643 | - | CCT CTGTAAATCGGATGACGTGT | |
| Cas9AtCBF2_081 | AtCBF2 | 4 | 13015760 | 13015782 | + | CGAGTCAGCGAAATTGAGAC AGG |
| AtCBF3 | 4 | 13018677 | 13018699 | + | CGAGTCAGCGAAATTGAGAC AGG | |
| AtCBF4 | 5 | 21117452 | 21117474 | + | AGAATCAGCGAAATTGAGAC AAG | |
| Cas9AtCBF2_123 | AtCBF2 | 4 | 13015754 | 13015776 | - | CCA AGCCGAGTCAGCGAAATTGA |
| AtCBF3 | 4 | 13018671 | 13018693 | - | CCA AGCCGAGTCAGCGAAATTGA | |
| AtCBF1 | 4 | 13022239 | 13022261 | - | CCA AGCCGAGTCAGCGAAGTTGA | |
| Cas9AtCBF2_124 | AtCBF2 | 4 | 13015759 | 13015781 | - | CCG AGTCAGCGAAATTGAGACAG |
| AtCBF3 | 4 | 13018676 | 13018698 | - | CCG AGTCAGCGAAATTGAGACAG | |
| AtCBF1 | 4 | 13022244 | 13022266 | - | CCG AGTCAGCGAAGTTGAGACAT | |