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. 2020 Oct 21;16(10):e1008946. doi: 10.1371/journal.ppat.1008946

Fig 4. Low pH initiates partial L1 capsid disassembly via a p120- and γ-secretase-independent mechanism.

Fig 4

A. HeLa cells transfected with scrambled siRNA were infected with WT HPV16.L2F (Luc). At the indicated time point post infection, cells were lysed and the resulting extract layered on top of a discontinuous 20–40% sucrose gradient. Following centrifugation, fractions were collected and subjected to SDS-PAGE and immunoblotting using antibodies recognizing L1 or FLAG-tagged L2. The levels of L1 in fractions 1–7 (disassembled) and fraction 8 (intact) were quantified. Data represent the mean ± SD of at least three independent experiments. B. (upper panels) HeLa cells were treated with bafilomycin A1 (BafA1) for 24 h, then infected, processed, and quantified as in A. Data represent the mean ± SD of at least three independent experiments. (lower panels) Purified WT HPV16.L2F (Luc) was treated with the chemical denaturant SDS, and the sample processed as in A. C. HeLa cells transfected with the indicated p120 siRNA were infected, processed, and quantified as in A. Data represent the mean ± SD of at least three independent experiments. D. HeLa cells transfected with the PS1 siRNA were infected, processed, and quantified as in A. Data represent the mean ± SD of at least three independent experiments.