Table 1. Cytolytic passaging of the SAT1 and SAT2 viruses in BHK-21 cells: the plaque morphologies and their titres in PFU/ml on BHK-21, CHO-K1, CHO-677, CHO-745 and CHO-Lec2 cells are indicated.
| Viruses | Cell lines and plaque morphology | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Low Passage Isolates§ | BHK-21 Adapted Isolates* | ||||||||
| SAT1 | BHK-21 | Plaque morphology# | BHK-21 | Plaque morphology | + Heparin (1mg/ml) %reduction in number of plaques¥ | CHO-K1 | CHO-677 | CHO-745 | CHO-Lec2 |
| KNP/148/91 | 4.5×104 | L, opaque | 1.2×108 | M, S, clear | 89 | 9×107 | 0 | 0 | 3.9×106 |
| KNP/41/95 | 8.1×104 | M, L, opaque | 2.7×107 | L, S, clear | 80 | 4.5××106 | 0 | 0 | 1.4×106 |
| ZIM/13/90 | 1.8×103 | M, opaque | 3.0×105 | L, S, clear | 91 | 1.5×105 | 0 | 0 | 6.5×104 |
| KEN/5/98 | 5.4×103 | M, L, opaque | 9.4×105 | L, S, clear | 93 | 1.4×105 | 0 | 0 | 2.3×103 |
| TAN/1/99 | 5.0×103 | L, opaque | 1.8×107 | S, clear | 95 | 7.6×105 | 0 | 0 | 2.9×104 |
| UGA/1/97 | 4.5×102 | M, opaque | 1.0×107 | M, S, clear | 92 | 1.7×106 | 3.5×105 | 6.4×103 | 1.3×105 |
| SUD/3/76 | 6.6×104 | L, opaque | 4.4×107 | L, S, clear | 97 | 3.4×106 | 0 | 0 | 8.0×106 |
| NIG/5/81 | 4.0×103 | M, opaque | 7.2×106 | L, S, clear | 78 | 7.2×105 | 0 | 0 | 1.6×104 |
| NIG/15/75 | 3.5×102 | M, opaque | 2.8×107 | L, S, clear | 82 | 1.8×106 | 0 | 0 | 3.5×105 |
| NIG/6/76 | 1.6×102 | M, opaque | 6.8×106 | L, S, clear | 98 | 8.6×105 | 0 | 0 | 1.2×105 |
| SAT2 | |||||||||
| KNP/2/89 | 1.0×105 | M, L, opaque | 1.0×107 | L, S, clear | 96 | 1.4×106 | 0 | 0 | 1.7×104 |
| KNP/51/93 | 2.9×103 | L, opaque | 8.6×105 | L, S, clear | 96 | 5.8×107 | 0 | 0 | 6.1×104 |
| ZIM/10/91 | 3.8×102 | L, opaque | 2.0×107 | L, S, clear | 96 | 1.4×106 | 0 | 0 | 5.6×104 |
| UGA/2/02 | 2.3×102 | L, opaque | 1.4×107 | M, S, clear | 69 | 2.1×106 | 1.6×105 | 1.0×104 | 6.6×105 |
| SAU/6/00 | 1.0×105 | M, opaque | 1.6×105 | L, clear | 0 | 0 | 0 | 0 | 0 |
§ The passage histories have been described in Maree et al. [38]. None of the low passage viruses grew on CHO-K1 cells.
# The plaque morphologies prior to BHK-21 cytolytic passage are depicted as L for large plaques, M for medium plaques and S for small plaques. Prior to cytolytic passage. Large plaques were defined as those with a diameter of 6–8 mm; medium plaques were 3–5 mm in diameter. Plaque sizes are based on three repeats of titrations and average measure of 30 plaques.
* Subsequent to cytolytic passage. Small plaques had a diameter of 1–3 mm.
¥ The average % reduction in the number of plaques on BHK-21 cells in the presence of 1mg/ml heparin from two experiments.