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. Author manuscript; available in PMC: 2021 Mar 13.
Published in final edited form as: Circ Res. 2020 Feb 13;126(6):737–749. doi: 10.1161/CIRCRESAHA.119.315760

Figure 5. Contractile oscillations in HO myocytes after TEVp treatment recorded during steady state force activations.

Figure 5.

A) Representative force traces from a single HO myocyte before TEVp treatment, after TEVp treatment, and after ligation of rC0C7-sc. Traces were arbitrarily shifted along the Y-axis for clarity. Force traces after TEVp treatment showed variations in steady state force due to underlying oscillatory contractions visible in contracting myocytes (see Online Video I). B) PIVLab image analysis of a contracting HO myocyte to represent force oscillations as changes in vector direction (black arrows) over time (inset, 1, 2, 3, 4) (see also Online Video I). White boxed region indicates area chosen for analysis in C. C) Results of speckle tracking analysis using Tracker 5.0.7 of 4 speckles in the white boxed region shown in B was performed and average displacement was plotted over time. Individual sarcomeres and contiguous groups of sarcomeres showed organized wave-like oscillations when activated at a constant pCa.