FIG. 4:

Phyto-β-SERM diet promoted expression of hippocampal proteins involved in neural plasticity and Aβ degradation/clearance. Three-month-old female mice were OVX or sham-OVX and fed one of the three test diets: a phytoestrogen-free control diet, a phyto-β-SERM formulation-containing diet or a commercial soy extract-containing diet, for 9 months. At the time of sacrifice, brain tissues were collected and dissected; hippocampus were processed and analyzed by Western blot for changes in expression levels of proteins associated with learning and memory. Data are presented as percent of phytoestrogen-free diet-treated sham-OVX control group and expressed as group mean ± S.E.M.; N / group = 5-7. * P < 0.05, ** P < 0.01 and *** P < 0.001.