Figure 3.

Spatial organization of NR and mPFC cell assemblies. A, Representation of the position of a linear silicon probe with 32 recording sites in (Aa) the NR and (Ab) mPFC. NR and mPFC contours (and layers) are delimited by the white dashed line over the green fluorescent Nissl staining. fmi, Forceps minor of the corpus callosum; third V, third ventricle; PrL, prelimbic area; IL, infralimbic area. Red-orange staining represents the DiI that was deposited at the back of the silicon probe before insertion. D, Dorsal; L, lateral. B, Relationship between anatomic dorsoventral location of NR (left) and mPFC (right) neurons (location defined by the site of the probe recording the maximum amplitude of the action potentials) and the template rank showing a linear correlation for NR neurons but not for mPFC ones in a template experiment. C, Distribution of spatial delays (from the beginning to end of a sequence) across the probe from the top-most site to the bottom-most one for mPFC (red) and NR (blue) neurons. D, Mean sequence delays (with median difference and 95% CI) across experiments for NR and mPFC. E, The percentage of experiments showing a dorsoventral (DV) orientation of sequences was larger in the NR than in the mPFC. (*p < 0.05).