Table 1. Biophysical and in vivo properties of Skp variants.
Error estimates have been omitted for clarity. n.d., not determined.
| C0.5 (μM) |
Skp activity (% of WT) |
Trimer (%) at c ≃ 80 μM |
Monomer (%) at c ≃ 80 μM |
Salmonella fitness in rich LB (% of WT) |
Salmonella fitness in mouse infection model (% of WT) |
|
| Skp(WT) | 1.5 | 100 | 97 | 3 | 100 | 100 |
| Skp(A103L) | 1.5 | 100 | 94 | 6 | 106 | 92 |
| Skp(A103R) | 7 | 87 | 86 | 14 | 102 | 58 |
| Skp(A108L) | 80 | 55 | 47 | 53 | n.d. | n.d. |
| Skp(A108R) | >1000 | 1 | 0 | 100 | n.d. | n.d. |
| Skp(V117P) | >1000 | 1 | 0 | 100 | 102 | 54 |
| ΔSkp | – | – | – | – | 104 | 54 |