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. 2020 Oct 21;6(43):eabb3063. doi: 10.1126/sciadv.abb3063

Fig. 6. rs708384 stimulates FAM171A2 expression and subsequently inhibits GRN/PGRN level.

Fig. 6

(A) The structure of firefly luciferase reporter plasmid. The sequence containing rs708384 was labeled by a red square. (B) The plasmids with or without rs708384 (c > a), including the empty control, had different expressing levels of firefly luciferase after transfection into the HEK293 cells. The luminous intensity was calibrated by Renilla luciferase, and the result was presented by ratio of firefly/Renilla luciferase. n = 4 per group, the data were analyzed by the one-way analysis of variance (ANOVA) (P < 0.0001) followed by the Tukey post hoc test (P = 0.0002 c > a versus wild type). (C and D) The change of intracellular GRN after FAM171A2 overexpression. n = 5 per group, the data were analyzed by t test (P = 0.0002). (E) The change of the supernatant PGRN levels by FAM171A2 overexpression (OE). n = 5 per group, the data were analyzed by t test (P < 0.0001).