Fig. 3. Single-shot 3D imaging of 10-μm fluorescent particles in a clear volume.

(A) xy MIP of the reconstructed volume spanning 5.7 mm by 6.0 mm by 1.0 mm. Top left inset: Raw CM² measurement. The FOV of the CM² is comparable to a 2× objective lens (red bounding box) and is ~25× wider than the 10× objective lens (blue bounding box). (B) Zoom-in of the CM² 3D reconstruction benchmarked by the axial stack taken by a 10×, 0.25 NA objective lens. (C) Lateral and axial cross sections of the recovered 10-μm particle. By comparing with the measurements from the standard wide-field fluorescence microscopy, the CM² faithfully recovers the lateral profile of the particle and achieves single-shot depth sectioning. A.U., arbitrary units. (D) xz cross-sectional view of a reconstructed fluorescent particle, as compared to the axial stack acquired from the 2× and 10× objective lenses. (E) To characterize the spatial variations of the reconstruction, the statistics of the lateral and axial FWHMs of the reconstructed particles are plotted for the central, outer, and corner FOV (as defined in Fig. 2C). The lateral width changes only slightly (~0.9%) in the outer FOV but increases in the corner FOV (~13.9%). The axial elongation degrades from ~246 μm in the central FOV to ~292 and ~ 299 μm in the outer and corner FOV regions, respectively.