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. 2020 Oct 21;6(43):eabc3175. doi: 10.1126/sciadv.abc3175

Fig. 6. Inhibition of collagen VI–driven signaling reduces TNBC cell migration on dECM derived from the mammary gland of obese and tumor-bearing mice.

Fig. 6

(A) Representative rose plots of 231-GFP cell migration seeded on dECM from obese mammary gland and treated with IgG2a or NG2ab, where each colored line represents the tracked movement of a cell over the time course (16 hours). (B) Cell migration speed of 231-GFP cells seeded on mammary gland ECM derived from lean mice, obese mice, and tumor-bearing mice treated with IgG2a or NG2ab. Graphs show mean ± SEM, with dot representing data from a single cell. Unpaired two-tailed Mann-Whitney tests were performed between respective IgG2a control and NG2ab conditions. Data were obtained from at least three different ECM scaffolds from three different mice, with an average of 108 total cells per condition. Significance by t test, with ***P < 0.005. (C) Representative rose plots of PyMT-GFP cell migration seeded on dECM from PyMT-tumors and treated with vehicle, NG2ab+ EGFRi or MAPKi, where each colored line represents the tracked movement of a cell over the time course (16 hrs) (D) Cell migration speed of PyMT-GFP cells seeded on mammary gland ECM derived from PyMT-mice treated with vehicle, NG2ab+ EGFRi or MAPKi. Data obtained from at least 3 different ECM scaffolds from 3 different mice, statistic by one-way Anova, with ***P < 0.005.