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. 2020 Oct 21;27(6):890–904.e8. doi: 10.1016/j.stem.2020.10.005

Figure 4.

Figure 4

SARS-CoV-2 Infection Induces Loss of Surfactants and AT2 Death

(A) Schematic for SARS-CoV-2-GFP infection in human alveolospheres. Alveolospheres were cultured in SFFF medium, infected with SARS-CoV-2 virus, and collected for histological analysis.

(B) Quantification of the percentage of SARS-CoV-2-infected alveolospheres.

(C) Immunostaining for GFP (green), SFTPC (red), and SARS (gray) (top panel) and GFP (green) and SFTPB (red) (bottom panel) in control, “low,” and “high” SARS-CoV-2-GFP-infected human lung alveolospheres at 72 h post-infection. Scale bar: 50 μm.

(D) Quantification of low-infected (1–10 SARS-CoV-2+ cells) and high-infected (10 or more SARS-CoV-2+ cells) alveolospheres.

(E) Quantification of SFTPC+ cells in uninfected control and SARS− and SARS+ cells in virus-infected alveolospheres.

(F) Immunostaining for GFP (green) in combination with the apoptotic marker active caspase 3 (red) and proliferation marker Ki67 (gray) in control and SARS-CoV-2-GFP-infected alveolospheres. Scale bar: 30 μm.

(G and H) Quantification of active caspase-3 (CASP3)+ (G) and Ki67+ (H) cells in uninfected control (gray), SARS-CoV-2− cells (blue), and SARS-CoV-2+ cells in infected alveolospheres. The white box in the merged image indicates the region of single-channel images. DAPI stains nuclei (blue). All quantification data are presented as mean ± SEM.