Table 2. Data collection and refinement statistics for 70S-ASLPro structures.
70S-ASLPro-CCG codon | 70S-ASLPro-CCC-U codon | |
---|---|---|
Data collection | ||
Space group | P212121 | P212121 |
Wavelength (Å) | 0.9791 | 0.9791 |
Cell dimensions | ||
a, b, c (Å) | 209.79,451.91,621.58 | 210.12,451.80,622.96 |
α, β, γ (°) | 90, 90, 90 | 90, 90, 90 |
Resolution (Å) | 49.70–3.10 (3.21–3.10) |
49.70–3.40 (3.52–3.40) |
Rpim (%) | 21.2 (80.5) | 14.5 (65.5) |
I/σI | 4.7 (1.1) | 5.9 (1.3) |
Completeness (%) | 97.99 (87.41) | 98.85 (94.87) |
Redundancy | 3.7 (3.1) | 4.2 (3.4) |
CC1/2 | 0.968 (0.250) | 0.987 (0.359) |
Refinement | ||
Reflections | 1034968 (91757) | 797600 (75973) |
Rwork/Rfree (%) | 24.1/27.2 | 19.8/23.7 |
No. atoms | 289313 | 290047 |
B-factors (Å2) | ||
Overall | 90.37 | 103.79 |
Macromolecule | 90.6 | 104.05 |
Ligand/ion | 39.43 | 43.84 |
R.m.s deviations | ||
Bond lengths (Å) | 0.004 | 0.010 |
Bond angles (°) | 0.83 | 0.98 |
PDB ID | 6NTA | 6NSH |
Highest resolution shell is shown in parentheses.