Table 3. Data collection and refinement statistics for 70S-tRNAPro structures.
Highest resolution shell is shown in parentheses.
| tRNAPro m1G37-CCG codon | tRNAPro m1G37-CCC-U codon | tRNAPro G37(Δm1)-CCG codon | tRNAPro G37(Δm1)-CCC-U codon | |
|---|---|---|---|---|
| Data collection | ||||
| Space group | P212121 | P212121 | P212121 | P212121 |
| Wavelength (Å) | 0.9792 | 0.9792 | 0.9792 | 0.9792 |
| Cell dimensions | ||||
| a, b, c (Å) | 210.20,451.47,620.21 | 210.74,450.26,626.11 | 209.97,450.71,619.40 | 210.09,450.32,622.89 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 |
| Resolution (Å) | 49.20–3.20 (3.31–3.20) |
49.93–3.50 (3.63–3.50) |
49.82–3.97 (4.11–3.97) |
49.83–4.14 (4.29–4.14) |
| Rpim (%) | 11.5 (51.7) | 9.00 (44.1) | 8.60 (86.0) | 9.807 (95.5) |
| I/σI | 6.7 (1.5) | 7.8 (1.7) | 6.3 (1.0) | 5.1 (1.0) |
| Completeness (%) | 99.11 (98.45) | 97.55 (89.72) | 98.60 (95.72) | 98.39 (95.63) |
| Redundancy | 5.9 (4.4) | 4.2 (2.2) | 14.1 (10.0) | 6.5 (3.6) |
| CC1/2 | 0.991 (0.377) | 0.996 (0.464) | 0.998 (0.313) | 0.998 (0.37) |
| Refinement | ||||
| Reflections | 951115 (93932) | 723555 (66052) | 4959167 (47742) | 439195 (42360) |
| Rwork/Rfree (%) | 22.8/25.6 | 23.4/25.6 | 22.8/25.5 | 24.8/29.4 |
| No. atoms | 291966 | 292039 | 291793 | 291185 |
| B-factors (Å2) | ||||
| Overall | 103.87 | 113.6 | 177.39 | 247.09 |
| Macromolecule | 104.12 | 113.9 | 177.77 | 247.54 |
| Ligand/ion | 42.04 | 37.71 | 72.42 | 123.12 |
| R.m.s deviations | ||||
| Bond lengths (Å) | 0.011 | 0.010 | 0.007 | 0.007 |
| Bond angles (°) | 1.00 | 1.23 | 0.94 | 1.38 |
| PDB ID | 6NUO | 6NWY | 6O3M | 6OSI |