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. 2020 Oct 21;9:e60058. doi: 10.7554/eLife.60058

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
Gene (Plasmodium falciparum) pfap2-g PlasmoDB PF3D7_1222600
Gene (Plasmodium falciparum) gexp02 PlasmoDB PF3D7_1102500
Cell line (Plasmodium falciparum) NF54-gexp02-Tom PMID:31601834 Maintained in culture with 2 mM choline
Cell line (Plasmodium
falciparum)
E5-gexp02-Tom PMID:31601834 Maintained in culture with 2 mM choline
Cell line (Plasmodium
falciparum)
NF54-10.3-Tom PMID:31601834 Maintained in culture with 2 mM choline
Commercial assay or kit RNeasy Mini Kit Qiagen Cat. No. 74104
Chemical compound, drug ML10 PMID:28874661; S. Osborne (LifeArc) and D. Baker (LSHTM) cGMP-dependent protein kinase inhibitor
Chemical compound, drug Dihydroartemisinin (DHA) Sigma-Aldrich Cat. No. D7439
Chemical compound, drug Chloroquine Sigma-Aldrich Cat. No. C6628
Chemical compound, drug Choline chloride Sigma-Aldrich Cat. No. C7527
Chemical compound, drug N-acetyl-d-glucosamine Sigma-Aldrich Cat. No. A8625
Software, algorithm BD FACSDiva Software BD Biosciences RRID:SCR_001456 Flow cytometryacquisition andanalysis using BD LSRFortessa machine
Software, algorithm Flowing Software version 2.5.1 Perttu Terho RRID:SCR_015781 Flow cytometry data analysis
Software, algorithm Prism 8 GraphPad RRID:SCR_002798
Antibody Pfs16 (mouse, monoclonal) R.Sauerwein, Radboud University 32F717:B02 IFA (1:400)
Antibody Goat-anti-mouse IgG–Alexa Fluor 488 Thermo Fisher Cat. No. A11029 IFA (1:1000)
Other SYTO 11 Life Technologies Cat. No. S7573 Flow cytometry (0.016 μM)
Other MitoTracker Deep Red FM Invitrogen Cat. No. M22426 Flow cytometry (0.6 µM)
Other DAPI Applichem lifescience Cat. No. A4099.0005 IFA (5 μg/mL)
Other Hoechst 33258 Thermo Fisher Cat. No. H3569 Live cellfluorescence microscopy (2 μM)