Key resources table.
Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
---|---|---|---|---|
Gene (Plasmodium falciparum) | pfap2-g | PlasmoDB | PF3D7_1222600 | |
Gene (Plasmodium falciparum) | gexp02 | PlasmoDB | PF3D7_1102500 | |
Cell line (Plasmodium falciparum) | NF54-gexp02-Tom | PMID:31601834 | Maintained in culture with 2 mM choline | |
Cell line (Plasmodium
falciparum) |
E5-gexp02-Tom | PMID:31601834 | Maintained in culture with 2 mM choline | |
Cell line (Plasmodium
falciparum) |
NF54-10.3-Tom | PMID:31601834 | Maintained in culture with 2 mM choline | |
Commercial assay or kit | RNeasy Mini Kit | Qiagen | Cat. No. 74104 | |
Chemical compound, drug | ML10 | PMID:28874661; S. Osborne (LifeArc) and D. Baker (LSHTM) | cGMP-dependent protein kinase inhibitor | |
Chemical compound, drug | Dihydroartemisinin (DHA) | Sigma-Aldrich | Cat. No. D7439 | |
Chemical compound, drug | Chloroquine | Sigma-Aldrich | Cat. No. C6628 | |
Chemical compound, drug | Choline chloride | Sigma-Aldrich | Cat. No. C7527 | |
Chemical compound, drug | N-acetyl-d-glucosamine | Sigma-Aldrich | Cat. No. A8625 | |
Software, algorithm | BD FACSDiva Software | BD Biosciences | RRID:SCR_001456 | Flow cytometryacquisition andanalysis using BD LSRFortessa machine |
Software, algorithm | Flowing Software version 2.5.1 | Perttu Terho | RRID:SCR_015781 | Flow cytometry data analysis |
Software, algorithm | Prism 8 | GraphPad | RRID:SCR_002798 | |
Antibody | Pfs16 (mouse, monoclonal) | R.Sauerwein, Radboud University | 32F717:B02 | IFA (1:400) |
Antibody | Goat-anti-mouse IgG–Alexa Fluor 488 | Thermo Fisher | Cat. No. A11029 | IFA (1:1000) |
Other | SYTO 11 | Life Technologies | Cat. No. S7573 | Flow cytometry (0.016 μM) |
Other | MitoTracker Deep Red FM | Invitrogen | Cat. No. M22426 | Flow cytometry (0.6 µM) |
Other | DAPI | Applichem lifescience | Cat. No. A4099.0005 | IFA (5 μg/mL) |
Other | Hoechst 33258 | Thermo Fisher | Cat. No. H3569 | Live cellfluorescence microscopy (2 μM) |