Figure 9.

Plots of disulfide formation for an IgG1 antibody and an IgG4 antibody on Protein A resin using a redox system containing 1 mM cysteine and 0.3 mM cystine at pH 8 and room temperature. (a) IgG1 mAb (mAb-1) with a starting purity of 57.6%; (b) IgG4 mAb (mAb-2) with a starting purity of 59.5%.. Each plot included six kinetic profiles, representing all six elementary reactions in the kinetic model (Scheme 1). Mono: intact mAb, L: light chain, H: heavy chain, HH: heavy-heavy fragment, HL: halfmer, HHL: heavy-heavy-light fragment.