Figure 10.

A model for the disulfide bond formation of mAb. (a) in solution. (b) on Protein A resin. A faster disulfide formation rate was observed on the solid surface compared to the reaction in solution. It was presumed that association of the reduced mAb and the solid surface led to an increase of rigidity of the molecule and a closer proximity of the free cysteines on the respective chains. The long blue bar represents the heavy chain, the short red bar represents the light chain, the orange bar represents the disulfide bond.