Table 5.
Validation of the kinetic model.
| Reoxidation Time (hrs) |
|||||||
|---|---|---|---|---|---|---|---|
| mAb Species | Initial composition (%) | Results (%) | 0.5 | 1.0 | 1.75 | 2.5 | |
| Sample 1 | H2L2 (Intact) | 14.0 | Predicted % | 52.5 | 83.3 | 91.1 | 93.8 |
| Actual % | 52.5 | 80.7 | 91.8 | 94.0 | |||
| HHL | 11.9 | Predicted % | 13.3 | 5.7 | 3.4 | 2.5 | |
| Actual % | 13.3 | 6.5 | 2.4 | 2.0 | |||
| HH | 3.8 | Predicted % | 1.4 | 0.8 | 0.5 | 0.4 | |
| Actual % | 1.4 | 0.5 | 0.2 | 0.3 | |||
| HL | 26.0 | Predicted % | 23.4 | 7.1 | 3.5 | 2.3 | |
| Actual % | 23.4 | 9.1 | 4.2 | 2.7 | |||
| H | 28.9 | Predicted % | 4.4 | 1.3 | 0.6 | 0.4 | |
| Actual % | 4.4 | 1.1 | 0.4 | 0.2 | |||
| L | 15.3 | Predicted % | 5.1 | 1.8 | 0.9 | 0.5 | |
| Actual % | 5.1 | 2.1 | 0.9 | 0.8 | |||
| Sample 2 | H2L2 (Intact) | 29.0 | Predicted % | 78.9 | 86.8 | 90.7 | 92.3 |
| Actual % | 72.9 | 89.1 | 94.0 | 95.9 | |||
| HHL | 19.1 | Predicted % | 6.6 | 4.8 | 3.9 | 3.6 | |
| Actual % | 9.1 | 3.3 | 1.6 | 1.1 | |||
| HH | 4.1 | Predicted % | 1.8 | 1.4 | 1.2 | 1.1 | |
| Actual % | 0.6 | 0.3 | 0.2 | 0.2 | |||
| HL | 24.6 | Predicted % | 8.5 | 4.8 | 2.8 | 2.0 | |
| Actual % | 12.6 | 5.8 | 3.4 | 2.1 | |||
| H | 12.6 | Predicted % | 2.3 | 1.4 | 1.0 | 0.8 | |
| Actual % | 1.8 | 0.4 | 0.1 | 0.1 | |||
| L | 10.7 | Predicted % | 1.9 | 0.9 | 0.4 | 0.2 | |
| Actual % | 3.0 | 1.2 | 0.7 | 0.6 | |||