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. 2020 Jun 5;25(11):2685–2694. doi: 10.1038/s41380-020-0801-x

Fig. 1. Maternal CAR exposure has a systemic effect on offspring with the most pronounced 7-DHC elevation in brain tissue.

Fig. 1

Samples were grouped taking into account the pups’ genotype and the levels of drugs were determined at P0. WT and Dhcr7-heterozygous pups are depicted in green or red, respectively. a Drug levels were determined in six different tissues and are presented as the sum of the parent drug and all active metabolites (CAR, DCAR, DDCAR, and 2,3-DCPP). b 7-DHC levels were measured across six different tissues of CAR-exposed pups and were found to be significantly higher in the Dhcr7+/− pups when compared with their WT littermates. c Levels of 7-DHC in different tissues of WT and Dhcr7+/− P0 pups after vehicle exposure; note that in all tissues measured values are significantly higher in the Dhcr7+/− pups when compared with their WT littermates. The levels of 7-DHC in the CAR-exposed pups are significantly higher than in the vehicle-exposed group (note the different scale on the Y-axis). A different representation of this figure is shown in the Supplementary Fig. 3, denoting individual values and sex of the pups. Bars correspond to the mean ± SEM; statistical significance: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.