Fig. 4. Elevated 7-DHC levels result in generation of toxic oxysterols at levels comparable to those seen in a Smith–Lemli–Opitz syndrome mouse model.
Levels of DHCEO, 4α-OH-7-DHC, and 4β-OH-7-DHC were determined in the brains P0 pups. Samples were grouped taking into account the pups’ genotype. a, c, and e denote the comparison of the three oxysterols between SAL- and CAR-exposed pups. b, d, and f denote the comparison between Dhcr7+/T93M (heterozygous) and Dhcr7Δ3-5/T93M (compound heterozygous SLOS model) pups. Each symbol corresponds to an individual pup brain; filled and opened symbols denote males and females, respectively; bars correspond to the mean ± SEM; statistical significance: *p < 0.05; **p < 0.01; ****p < 0.0001.