Figure 5. Inflammatory cell recruitment and cytokine secretion in thioglycollate-induced peritonitis.

A-B) Cell counts of neutrophils (A) and macrophages (B) in the peritoneum of CD11b^WT and CD11b^I332G mice by flow cytometry at 4 and 48 hours after sterile thioglycollate injection. Neutrophils were selected as Gr-1^hi, while macrophages were identified as F4/80⁺. Bars represent the mean ± SEM, n=7-9 mice per group. C-E) Secretion of inflammatory cytokines by peritoneal macrophages isolated at 4 days after thioglycollate injection. Cells were count normalized and stimulated with LPS for increasing periods of time in vitro. Bars represent the mean ± SEM, n=4 mice per group in three independent experiments. N.S., not significant.