Figure 2: Sleep patterns are disrupted in AS model mice.

A. Total sleep or wake in wild type (Ube3a^m+/p+) or AS (Ube3a^m-/p+) mice recorded over an uninterrupted 24 h period in 12 h light/dark cycle. Data are shown as percentage of each 2 h block spent asleep or awake within each mouse, averaged within genotypes (mean+/−SEM; n=6 wild type or 8 AS mice). Note the difference in sleep/wake amounts between genotypes across the night (indicated by the gray shading). B. AS model mice exhibit reduced nighttime sleep-pressure accumulation. Left - NREM sleep intensity, as measured by delta power, is significantly reduced in Ube3a^m-/p+ mice during the latter portion of the night. * = p<0.05 between genotypes, n= 6–8 mice. Right - The increase in the wave-incidence (I2– 6) across the night (wake period) is abolished in Ube3a^m-/p+ mice. Representative EEG recordings (black and red) are shown above bandpass-filtered versions (gray and dark red) of the same EEG recording. The incidence of peaks in the upper 30% by amplitude (blue line) in the filtered signal were counted in epochs scored as waking are indicated by arrowheads. I2– 6 has been previously validated as a measure of sleep-pressure accumulation in awake mice⁸². I2– 6 significantly increased across the active period in wild type (* = p<0.05) but not Ube3a^m-/p+mice. All data are replotted from Ehlen et al, 2015³⁶.