Extended Data Fig. 2. Flow cytometry gating strategy to define changes in T_reg cell phenotype in arrayed Cas9 RNP screen.

(a) Initial gating strategy to identify live cells. (b) Gating strategy to assess T_reg cell stability for personality and Scaffold plots. Control non-targeting Cas9 RNP-treated T_reg cells (ctrl) after IL-12 conditioning are shown in light blue and FOXP3 KO T_reg cells after IL-12 stimulation are shown in orange. a, b: Representative result for one non-targeting control Cas9 RNP in one out of two human blood donors. (c) % of live cells based on flow cytometry staining for all conditions tested in the arrayed Cas9 RNP screen.