Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 21;10:17885. doi: 10.1038/s41598-020-74628-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Generation and validation of Prcd-KO animal model. (A) Scheme demonstrating the generation of the Prcd-KO animal model using CRISPR/Cas9 technology. In this study, we used the 1738 founder line, which has a single base pair deletion in exon 1 of Prcd. UTR = untranslated region, CDS = coding sequence. (B) Immunoblot analysis shows loss of PRCD protein from Prcd-KO retinal lysate at postnatal (P) 30 and P100, whereas wild-type (WT) retinal lysates demonstrate PRCD immunoreactivity (n = 4). Please note that immunoblot data is cropped; full-length, raw data is available in Supplementary Fig. S2. (C) Immunofluorescent staining of P30 retinal cross-sections from WT and Prcd-KO mice, probing with antibodies against PRCD (red) and the OS marker cyclic nucleotide gated channel alpha-1 and alpha-3 (CNGA 1/3; green) to demonstrate proper localization of PRCD to the OS in WT retina and loss of PRCD from Prcd-KO retina (n = 3). Scale bar = 20 μm. All experiments were conducted with littermate controls.