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. 2020 Oct 21;10:17916. doi: 10.1038/s41598-020-73544-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Methodological procedure to produce the ontology. (A) Gametes were collected from adult individuals of C. robusta for in vitro fertilization. At a specific time, samples were observed via stereomicroscope, photographed, and fixed for CLSM and histology. Scale bar 2 cm. (B) Summary of Stages 26–37. Stereomicroscopy—in vivo specimens. In individuals belonging to Stages 34–36 (Body Axis Rotation Period), the blue line indicates the longitudinal body axes (the antero-posterior axis) that is parallel to the endostyle; the red line indicates the oral siphon-gut axis; the black line indicates the stalk axis. In an individual at Stage 37 (Juvenile Period), the rotation is almost completed and the longitudinal body axis is almost parallel to the stalk axis. Stages 26–33: anterior at left, left view; Stages 34–37: oral siphon (anterior) indicated by arrowhead; left view. Scale bar 100 μm. (C) After definition of the developmental stages, specimens and literature were analyzed. After that, entities in hierarchical order, definitions, synonyms, developmental information, and literature were annotated in an Excel file. These data were edited using OBO-Edit (https://oboedit.org/), allowing for the visualization of relationships among entities. (D) Lastly, data were associated with images and movies in the web-based resource TunicAnatO (https://www.bpni.bio.keio.ac.jp/tunicanato/3.0/).