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. 2020 Oct 21;10:17916. doi: 10.1038/s41598-020-73544-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Early body axis rotation (Stage 34). (A–A^IV) Metamorphosing larva seen from the left side (A) and its medial sagittal optic section (A^I). In A^I, the area bordered by the red line is enlarged in A^II; that one bordered by the black line is enlarged in A^III; the line marked by A^IV represents the level of section A^IV. Enlargement is the same in A–A^I, and in A^II–A^IV. CLSM. (B–B^IV) Serial sagittal histological sections of a metamorphosing larva from the left to right sides (B, B^II–B^IV); the area bordered by the black line in B is enlarged in B^I to show the oral siphon and neural complex. Enlargement is the same in B and B^II–B^IV. Toluidine blue. brc: branchial chamber; cil duc: ciliated duct of the neural gland; cg: cerebral ganglion; es: endostyle; hc: maemocytes; ht: heart; las: left atrial siphon; int: intestine; lbr: larval brain remnants; ng: neural gland; oes: oesophagus; os; oral siphon; osm: oral siphon muscle; pyc: pyloric caecum; ras: right atrial siphon; stom: stomach; tail remn: tail remnants; tun: tunic; tunc: tunic cells.