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. 2020 Oct 21;11:5321. doi: 10.1038/s41467-020-19173-2

Fig. 3. p53 mediates 5-FU-induced activation of the WNT/β-catenin signaling pathway.

Fig. 3

a Immunoblots of indicated proteins in various CRC cell lines treated with or without 5-FU (1.5 μg/ml, 48 h). b Confocal images of immunofluorescence staining of HCT116 cells treated with or without 5-FU and the mean intensity of β-catenin in p53low and p53high cells. Scale bar = 20 μm. (p53low n = 7, p53high n = 6 biologically independent samples). c Immunoblots of indicated proteins in p53+/+, p53−/−, and p53R248W/− isogenic HCT116 cell lines treated with or without 5-FU. d Immunoblots of indicated proteins in p21+/+ and p21−/− isogenic HCT116 cell lines treated with or without 5-FU. e Immunoblots of indicated proteins in HCT116 cells treated with or without treatment of 5-FU and pifithrin-α (10 μM, 48 h). f Immunoblots of indicated proteins in HCT116 cells treated with or without RITA (5 μM, 48 h). g, h Hematoxylin and eosin staining and immunofluorescence staining of β-catenin in ApcMin/+/Lgr5EGFP intestinal tumor organoids treated with or without 5-FU and pifithrin-α (g) and with or without RITA (h). Scale bar = 50 μm. The mean intensities were measured by Zen software 3.1. (n = 6 biologically independent samples per group) Data are mean ± s.d., two-sided Student’s t-test, n.s. not significant, *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source data file.