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. 2020 Oct 21;11:5321. doi: 10.1038/s41467-020-19173-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Relative mRNA levels of Wnt3 in Apc^Min/+/Lgr5^EGFP mouse tumor organoids after treatment with 5-FU (1.5 μg/ml, 48 h). (n = 3 biologically independent samples per group). b Relative mRNA levels of WNT3 in various CRC cell lines after 5-FU treatment. (n = 3 biologically independent samples per group). c Relative mRNA levels of WNT3 in p53^+/+, p53^−/−, and p53^R248W/− isogenic HCT116 cell lines after 5-FU treatment. (n = 3 biologically independent samples per group). d Relative mRNA levels of WNT3 in p21^+/+ and p21^−/− isogenic HCT116 cell lines after 5-FU treatment. (n = 3 biologically independent samples per group). e Relative mRNA levels of WNT3 in HCT116 cells treated with or without 5-FU and pifithrin-α (10 μM, 48 h). (n = 3 biologically independent samples per group). f Relative mRNA level of WNT3 in HCT116 cells treated with our with RITA (5 μM, 48 h). (n = 3 biologically independent samples per group). g Relative percentage input of ChIP-qPCR analysis of WNT3 promoter using indicated antibodies in HCT116 cells after treatment with 5-FU. (n = 3 biologically independent samples per group) h Confocal images of WNT3 (green) and β-catenin (green) with DAPI in APC^KO/p53^WT and APC^KO/p53^KO human colon organoids treated with or without 5-FU. Scale bar = 50 μm. Quantification of mean intensities of WNT3 and β-catenin in Fig. 4h. The mean intensities were measured by Zen software 3.1. (n = 3 biologically independent samples per group) Data are mean ± s.d., two-sided Student’s t-test, n.s. not significant, *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source data file.