KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Anti-GFP (Green Fluorescent Protein) pAb | MBL International | Cat#598; RRID:AB_591819 |
| Goat Anti-rabbit HRP | Pierce | Cat#1858415 |
| Bacterial and Virus Strains | ||
| E. coli: Alpha-Select Silver | Bioline | Cat#BIO-85026 |
| E. coli: BL21(DE3) | Novagen | Cat#69387-3 |
| E. coli: XL1-Blue | Stratagene | Cat#200249 |
| E. coli: BTH101 | EuroMedex | Cat#EUB001 |
| E. coli: DAP (diaminopimelic acid) auxotroph used for conjugation | W. Metcalf, Univ. of Illinois, Urbana [58] | WM3064 |
| Asticcacaulis biprosthecum C19 | [70] | ATCC: 27554 |
| Mutant strains, see Table S3 | ||
| Deposited Data | ||
| C. crescentus bacA/BacA gene and protein sequences | Integrated Microbial Genomes: https://img.jgi.doe.gov | Genome Name: Caulobacter crescentus CB15; Genome ID: 637000061; Gene ID: 637087916; Amino acids: 161 |
| C. crescentus bacB/BacB gene and protein sequences | Integrated Microbial Genomes: https://img.jgi.doe.gov and [12] | Genome Name: Caulobacter crescentus CB15; Genome ID: 637000061; Gene ID: 637089074; Amino acids: 180 |
| Asticcacaulis biprosthecum bacA/BacA gene and protein sequences | Integrated Microbial Genomes: https://img.jgi.doe.gov | Genome Name: Asticcacaulis biprosthecum C19, ATCC 27554 Genome ID: 651285000 Gene ID: 651319016 Amino acids: 181 |
| Structural coordinates of C. crescentus BacA | [51] | PBD 2N3D |
| Oligonucleotides | ||
| Primers for construct generation and sequencing, see Table S4 | Eurofins | N/A |
| Recombinant DNA | ||
| pMR10 | R. Roberts and C. Mohr | N/A |
| pNPTS138 | M.R.K. Alley | N/A |
| pNPTS139 | M.R.K. Alley | N/A |
| pGFPC-1 | [59] | N/A |
| pVENC-4 | [59] | N/A |
| pKT25 | EuroMedex | Cat#EUP-25C |
| pKNT25 | EuroMedex | Cat#EUP-25N |
| pUT18 | EuroMedex | Cat#EUP-18N |
| pUT18C | EuroMedex | Cat#EUP-18C |
| pKT25-zip | EuroMedex | Cat#EUP-25Z |
| pUT18C-zip | EuroMedex | Cat#EUP-18Z |
| pET28a+ | Novagen | Cat#69864 |
| Cloned vectors, see Table S2 | ||
| Software and Algorithms | ||
| Jalview | [61] | https://www.jalview.org/ |
| SWISS-MODEL | [50] | https://swissmodel.expasy.org/ |
| ESPript | [62] | http://espript.ibcp.fr/ESPript/ESPript/ |
| FIJI | [64] | https://fiji.sc/ |
| MicrobeJ | [65] | https://www.microbej.com/ |
| TrackMate | [66] | https://imagej.net/TrackMate |
| R | [67] | https://www.r-project.org/ |
| ggplot2 | [68] | https://cran.r-project.org/package=ggplot2 |
| ggsignif | [69] | https://cran.r-project.org/package=ggsignif |